I’ve had hard time figuring out that different website and file formats are using different systems to represent genome coordinate.
Basically, the bases can be numerated in two way: starting at 0 or starting at 1. Those are the 0-based and 1-based coordinate system.
Then you say that the system is inclusive if the last index is part of the sequence or exclusive if it is not.
For instance to represent the sequence TGAC:
0-based inclusive: 2-5
1-based inclusive: 3-6
1-based exclusive: 3-7
I’ve tried to figure out which website-application are using each coordinate system. The results can be found bellow. For each source, I provide the URL of the reference website where I found the information, and a caption where the system is described.
I found most of those links in Biostar (https://www.biostars.org/p/6373/) and on the blog of Casey M. Bergman (http://bergmanlab.smith.man.ac.uk/?p=36), who also wrote an article about this argument: https://www.landesbioscience.com/journals/mge/article/19479/.
- Ensembl: 1-based inclusive, ref: http://www.ensembl.org/Help/Faq?id=286)(http://www.ensembl.org/info/docs/api/core/core_tutorial.html
Ensembl, and many other bioinformatics applications, use inclusive coordinates which start at 1. The first nucleotide of a DNA sequence is 1 and the first amino acid of a protein sequence is also 1. The length of a sequence is defined as end – start + 1.
(Ensembl gtf format = gff2 = 1-based)
- UCSC: internal representation: 0-based start and 1-based end, display: 1-based, ref: http://www.ensembl.org/Help/Faq?id=286) http://genome.ucsc.edu/FAQ/FAQtracks.html#tracks1
“I am confused about the start coordinates for items in the refGene table. It looks like you need to add “1” to the starting point in order to get the same start coordinate as is shown by the Genome Browser. Why is this the case?”Response:
Our internal database representations of coordinates always have a zero-based start and a one-based end. We add 1 to the start before displaying coordinates in the Genome Browser. Therefore, they appear as one-based start, one-based end in the graphical display. The refGene.txt file is a database file, and consequently is based on the internal representation.
We use this particular internal representation because it simplifies coordinate arithmetic, i.e. it eliminates the need to add or subtract 1 at every step. Unfortunately, it does create some confusion when the internal representation is exposed or when we forget to add 1 before displaying a start coordinate. However, it saves us from much trickier bugs. If you use a database dump file but would prefer to see the one-based start coordinates, you will always need to add 1 to each start coordinate.
If you submit data to the browser in position format (chr#:##-##), the browser assumes this information is 1-based. If you submit data in any other format (BED (chr# ## ##) or otherwise), the browser will assume it is 0-based. You can see this both in our liftOver utility and in our search bar, by entering the same numbers in position or BED format and observing the results. Similarly, any data returned by the browser in position format is 1-based, while data returned in BED format is 0-based.
- Bed format: 0-based exclusive, ref: http://genome.ucsc.edu/FAQ/FAQformat.html#format1
BED format uses zero-based, half-open coordinates, so the first 25 bases of a sequence are in the range 0-25 (those bases being numbered 0 to 24)
The first three required BED fields are:
- MAF: 1-based inclusive, ref: https://wiki.nci.nih.gov/display/TCGA/Mutation+Annotation+Format+%28MAF%29+Specification+-+v2.4